12
phenolphthalein indicator until a red color appeared. The slide shows the filtrate before (left) and
after (right) titration. The acidity of raw materials was determined by the formula; it amounted to
11.3 degrees for dietary supplements and 8.3 degrees for thyme.
4 Results, processing:
X=
)
100
(
100
100
100
0
0
W
m
D
m
D
x
x
,D
x
- the optical
density of the test solution;D
0
-optical density of the
RSO routine; (obtained from the teacher); m
x
- mass of raw materials (g);m
0
- mass of rutin in PCO
(0.035 g);W - moisture content in plant raw materials (%)
Based
on the results obtained, a table is drawn up and the optimal time for the extraction of
flavonoids is determined.
Table 3.determination of flavonoidsdetermination of flavonoids in thyme and BAS
Sample
Х, %
DS 30 min
0,39
DS 45 min
0,40
Thyme 30 min
0,75
Thyme 45 min
0,88
Figure 1.Absorption Spectra of Serpylliherba Plant Extracts and Sedative Collection
The amount of flavonoids was determined. To do this, the plant material was extracted with
alcohol, the first part of the extracts was kept for 30 minutes, the second - 45 minutes. The
absorption spectra were filtered and recorded on a Specord 50 instrument. The wavelength
corresponding to the absorption maximum of flavonoids was selected. The amount of flavonoids
calculated in terms of rutin was calculated. The results show that the optimal extraction time is 45
minutes. Moreover, the content of flavonoids in the thyme is greater than in dietary supplements.
In Russia, the content of flavonoids in plants of the genus Thymus (including T. serpyllum
and T. vulgaris widely used in official medicine) has been little studied.
The sum of flavanoids according to volume 3 of the 2013 Pharmacopoeia is considered in
terms of luteolin-7-O-glucoside.
In the article validation of methods of quantitative determination of the amount of flavonoids in the
trees of the thyme scientific reports belsu series medicine.pharmacy. 2012. no. 22 (141).issue 20/1
there is data on the content of the total flavanoid in terms of luteolin 1.34% and cinaroside 0.3 mg.
13
the total content of quercetin is 3.54% in the article
features of accumulation of
pharmacologically significant compounds in representatives of the genus thymyan (thymus l.)
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