Студенттер мен жас ғалымдардың «фараби әлемі» атты халықаралық ғылыми конференциясы

«Фараби Әлемі» атты студенттер мен жас ғалымдардың халықаралық конференциясы
31 
STANDARTIZATION OF ESSENTIAL OIL FROM MELISSA OFFICINALIS L. ON THE 
CONTENT OF CARYOPHYLLENE 
Amangeldi A.N. 
Scientific supervisor: c.c.s., senior lecturer Miftakhova A.F.
Al-Farabi Kazakh National University 
Alnura.a.2002@gmail.com

 
The purpose of research work is relying on the hydro-distillation method using Clevenger 
apparatus get the essential oil and offer the new way standardization process for received product 
from the Melissa officinalis L.
Nowadays, the standardization of Melissa officinalis L. essential oil on the content of 
caryophyllene not yet proposed according to the State Pharmacopoeia of the Republic of 
Kazakhstan. In this study a number of works have been done to obtain and standardize essential oil 
from natural product for the content of caryophyllene oxide. In the Clevenger device, sample-mixed 
water is heated to a high temperature and then boiled to remove volatile components. Oil-rich layers 
are produced and the essential oil separated.
Numbers of experiments like quantitative test with chromatographic method, tests on density 
of product, test on solubility and refractive index were done for standatization of essential oil of 
Melissa officinalis L. for the content of caryophyllene. For gas chromatography the Agilent 
6890/5973 chromato-mass spectrometer was used. After completing the process, some natural 
products like 2,6-Octadienal, 3, 7 - dimethyl- , caryophyllene oxide, 2,6-Octadien-1-ol, 3,7-
dimethyl- were detected in the structure of Melissa officinalis L.. This can be proved by some 
spotted intensive peaks on the obtained graph. These results show that Melissa officinalis L. contain 
these natural products. Overall, 9 peaks were detected which proves authenticity of our plant 
material and optimized scheme of production of essential oil.
Caryophyllene C
15
H
24
is a terpene hydrocarbon. It is colorless oily liquid with a sharp woody 
smell. It is soluble in nonpolar organic solvents, insoluble in water. Caryophyllene is used to 
compose perfume compositions, in the production of perfumes for soap, cosmetic products, in the 
synthesis of some fragrant substances and also used as anti-inflammatory and anti-stress component 
in some biologically active additives. 
The results of the experiment can be used in other study works as authentic resource and in 
projects which require the scheme of production and standartization of essential oils from Melissa 
officinalis L. for the content of caryophyllene. 

«Фараби Әлемі» атты студенттер мен жас ғалымдардың халықаралық конференциясы
32 

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