Клиническая медицина Медицина и экология, 2021, 3

22 
Клиническая медицина 
increased body length (3-6
th
intervals of the 
scale) and a harmonious ratio of length/body 
weight (4-5
th
intervals of the nomogram). 
The concept of «risk group» for physical 
development included children: 
• 
with low, high and very high body 
length (2,7,8
th
scale intervals) with harmonious 
development; 
• 
with disharmonious physical develop-
ment due to deficiency or excess body weight (2-
3
rd
or 6-7
th
intervals of nomograms) with a body 
length estimated within 2-8
th
intervals of the 
scale [20]. 
The group with disabilities in physical 
development corresponded to: 
1. Very low body length (1
st
scale inter-
val) at any body weight 
2. Sharply disharmonious development 
due to body weight deficiency (1
st
interval of the 
nomogram) at any body length 
3. Sharply disharmonious development 
due to excess body weight (8
th
interval of the 
nomogram) at any body length [21]. 
After the distribution of children into 
groups of physical development, a laboratory ex-
amination was carried out in 132 adolescents 
(main group 89, control group 43), the program 
of which included: determination of calcium, inor-
ganic phosphorus, PTH, calcitonin, osteocalcin in 
blood serum. In order to assess the features of 
bone tissue remodeling, P1NP and 
β
-Crosslaps 
were determined in blood serum. 
The material of the study in both groups 
of observation was venous blood, which was 
placed in a sterile centrifuge tube without antico-
agulants. The samples were frozen at a tempera-
ture of –
75 
°C. All samples were analyzed in du-
plicate in accordance with the requirements of 
the analysis. 
To determine the concentration of P1NP, 
the method of electrochemiluminescence immu-
noassay, Cobas e601 (Roche) was used; 
β
-
Crosslaps –
by immunoassay, Osteocalcin-
electrochemiluminescence immunoassay «sand-
wich» 2-site immunoassay, specific for intact os-
teocalcin (1 –
49) 
and a fragment (1 –
43); 
Ca-
colorimetric photometric method, P-colorimetric 
with ammonium molybdate; calcitonin –
solid 
phase chemiluminescent enzyme immunoassay. 
Statistical data processing was carried out using: 
1) descriptive statistics of quantitative 
data in groups, 
2) comparison of groups on a personal 
computer using MS Excel, EpiData database, 
3) the licensed statistical program SAS 
9.2. 
The following indicators were calculated: 
mean, median, mode, standard error of the 
mean, standard deviation. A p level value <0.05 
was considered statistically significant. 

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